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Wednesday, April 22 • 9:40am - 10:00am
Characterization of Genetic Diversity in Demes of Purple Pitcher Plants (Sarracenia purpurea L.) from Western North Carolina

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Purple pitcher plant (Sarracenia purpurea L.) is a carnivorous species that is widespread throughout the coastal plain of eastern North America. In western North Carolina, S. purpurea var. montana (mountain purple pitcher plant) exhibits a much more limited distribution and is found only in isolated montane bogs and fens, which serve as ecosystem islands. Population sizes in mountain bogs are fairly small, ranging from as few as 2 up to approximately 300 individuals. In addition, many bogs are spatially isolated, with S. purpurea var. montana located up to 14 km from the next known population. We worked to characterize the genetic structure of eight S. purpurea var. montana demes in Western North Carolina using microsatellite loci. DNA was extracted from leaf tissue using Qiagen kits, and 5 microsatellite primer sets were used to amplify polymorphic regions of the genome. PCR products were run through agarose gel electrophoresis to visualize and quantify band sizes. Data from this project will help the United States Fish and Wildlife Service determine S. purpurea var. montana’s suitability as a candidate species for conservation and preservation programs.

Wednesday April 22, 2015 9:40am - 10:00am PDT
014 Zeis Hall

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